Journal: Blood

Article Title: Critical role of scavenger receptor-BI-expressing bone marrow-derived endothelial progenitor cells in the attenuation of allograft vasculopathy after human apo A-I transfer.

doi: 10.1182/blood-2008-06-161794

Figure Lengend Snippet: Figure 1. Human apo A-I gene transfer and EPC number in chimeric C57BL/6 SR-BI/ mice and chimeric C57BL/6 SR-BI/ mice. (A) Human apoA-I expression levels after adenoviral gene transfer with 5 1010 particles of AdA-I in male C57BL/6 mice transplanted with SR-BI/ (F; n 10) or SR-BI/ (Œ; n 10) bone marrow 4 weeks before gene transfer. (B) Time course of the number of Flk-1 Sca-1 double-positive cells in AdA-I (F, Œ)– or Adnull (E, ‚)–treated C57BL/6 mice with SR-BI/ (F, E) or SR-BI/ (Œ, ‚) bone marrow (n 10 for each group). (C) Bar graph showing the number of DiI-acLDL FITC-isolectin double-positive cells after 7 days of ex vivo culture of bone marrow mononuclear cells isolated at day 35 after Adnull transfer or AdA-I transfer in C57BL/6 mice with SR-BI/ or SR-BI/ bone marrow (n 10 for each group). (D) Bar graph showing the number of Flk-1 Sca-1 double-positive cells in the bone marrow of C57BL/6 mice with SR-BI/ or SR-BI/ bone marrow at day 35 after transfer with Adnull or AdA-I. Data are expressed as number/10 000 mononuclear cells (MNC) (n 10 for each group). Data are mean plus or minus SEM.

Article Snippet: After overnight incubation with a 1:1000 dilution of rabbit anti–mouse SR-BI primary antibody (Abcam, Cambridge, United Kingdom) and subsequent incubation for 1 hour with a horseradish peroxidase–conjugated goat anti–rabbit antibody (Dako Denmark, Glostrup, Denmark) in a 1:100 dilution, a positive signal was detected using the NEL 704 Kit (PerkinElmer Life and Analytical Sciences, Waltham, MA) according to the instructions of the manufacturer.

Techniques: Expressing, Ex Vivo, Isolation

Journal: Blood

Article Title: Critical role of scavenger receptor-BI-expressing bone marrow-derived endothelial progenitor cells in the attenuation of allograft vasculopathy after human apo A-I transfer.

doi: 10.1182/blood-2008-06-161794

Figure Lengend Snippet: Figure 2. SR-BI and signal transduction induced by HDL in EPCs. (A) Bar graph showing the number of migrated EPCs in modified Boyden chambers. After 7 days of culture, bone marrow EPCs isolated from control mice or AdA-I–treated C57BL/6 mice with SR-BI/ or SR-BI/ bone marrow were seeded in the upper chamber. The lower chamber was supplemented with either HDL (100 g/mL) or an equivalent amount of bovine serum albumin and the number of migrated cells per microscopy field was quantified after 5 hours (n 4 for each group). (B) Bar graph showing the number of migrated EPCs in modified Boyden chambers. After 7 days of culture, EPCs isolated from chimeric SR-BI/ C57BL/6 mice (n 6) were seeded in the upper chamber. The lower chamber was supplemented with either bovine serum albumin (100 g/mL) or HDL (100 g/mL). To inhibit ERK signaling and NO synthase activity, experiments were performed in the presence of U0126 (10 M) and LNMA (2 mM), respectively. The number of migrated cells per microscopy field was quantified after 5 hours (n 6 for each group). (C) p-ERK/ERK ratio determined by Western blot. After 7 days of culture, bone marrow EPCs isolated from SR-BI/ or SR-BI/ mice were exposed to either bovine serum albumin (100 g/mL) or HDL (100 g/mL) for 2.5 minutes (n 4 for each group). (D) NO production in cultured bone marrow EPCs. After 7 days of culture, bone marrow EPCs isolated from SR-BI/ or SR-BI/ mice were exposed to either bovine serum albumin (100 g/mL) or HDL (100 g/mL) for 24 hours, and NO production (nanomoles per milligram of protein) was measured (n 5 for each group). (E) NO production in cultured EPCs. After 7 days of culture, EPCs isolated from chimeric SR-BI/ C57BL/6 mice (n 4) were exposed to either bovine serum albumin (100 g/mL) or HDL (100 g/mL). To inhibit ERK signaling, experiments were performed in the presence of U0126 (10 M). NO production (nanomoles per milligram of protein) was determined (n 4 for each group). (F) NO production in the bone marrow at day 35 after transfer with Adnull or saline (Controls) or AdA-I in C57BL/6 mice with SR-BI/ (n 8 for each group) or SR-BI/ bone marrow (n 18 for each group). Data are mean plus or minus SEM.

Article Snippet: After overnight incubation with a 1:1000 dilution of rabbit anti–mouse SR-BI primary antibody (Abcam, Cambridge, United Kingdom) and subsequent incubation for 1 hour with a horseradish peroxidase–conjugated goat anti–rabbit antibody (Dako Denmark, Glostrup, Denmark) in a 1:100 dilution, a positive signal was detected using the NEL 704 Kit (PerkinElmer Life and Analytical Sciences, Waltham, MA) according to the instructions of the manufacturer.

Techniques: Transduction, Isolation, Control, Microscopy, Activity Assay, Western Blot, Cell Culture, Saline

Journal: Blood

Article Title: Critical role of scavenger receptor-BI-expressing bone marrow-derived endothelial progenitor cells in the attenuation of allograft vasculopathy after human apo A-I transfer.

doi: 10.1182/blood-2008-06-161794

Figure Lengend Snippet: Figure 3. Effect of human apo A-I transfer on allograft vasculopathy in chimeric C57BL/6 SR-BI/ mice and chimeric C57BL/6 SR-BI/ mice. Representative sec- tions of allograft vasculopathy at day 21 after transplanta- tion in control (A,C) and AdA-I (B,D)–treated C57BL/6 mice with SR-BI/ (A,B) or SR-BI/ (C,D) bone marrow.

Article Snippet: After overnight incubation with a 1:1000 dilution of rabbit anti–mouse SR-BI primary antibody (Abcam, Cambridge, United Kingdom) and subsequent incubation for 1 hour with a horseradish peroxidase–conjugated goat anti–rabbit antibody (Dako Denmark, Glostrup, Denmark) in a 1:100 dilution, a positive signal was detected using the NEL 704 Kit (PerkinElmer Life and Analytical Sciences, Waltham, MA) according to the instructions of the manufacturer.

Techniques: Control

Journal: Blood

Article Title: Critical role of scavenger receptor-BI-expressing bone marrow-derived endothelial progenitor cells in the attenuation of allograft vasculopathy after human apo A-I transfer.

doi: 10.1182/blood-2008-06-161794

Figure Lengend Snippet: Figure 4. Effect of human apo A-I transfer on endothe- lial regeneration and EPC incorporation in chimeric C57BL/6 SR-BI/ mice and chimeric C57BL/6 SR- BI/ mice. The effect of AdA-I transfer on endothelial cell regeneration in allografts is dependent on SR-BI expression in bone marrow–derived cells. (A) Bar graph showing the number of CD31 endothelial cells at day 21 after artery transplantation in control and AdA-I–treated C57BL/6 mice with SR-BI/ or SR-BI/ bone marrow. (B) Bar graph showing the number of CD31 Y-chromosome–positive endothelial cells at day 21 after artery transplantation in control and AdA-I–treated C57BL/6 mice with SR-BI/ or SR-BI/ bone marrow. Data are mean plus or minus SEM.

Article Snippet: After overnight incubation with a 1:1000 dilution of rabbit anti–mouse SR-BI primary antibody (Abcam, Cambridge, United Kingdom) and subsequent incubation for 1 hour with a horseradish peroxidase–conjugated goat anti–rabbit antibody (Dako Denmark, Glostrup, Denmark) in a 1:100 dilution, a positive signal was detected using the NEL 704 Kit (PerkinElmer Life and Analytical Sciences, Waltham, MA) according to the instructions of the manufacturer.

Techniques: Expressing, Derivative Assay, Transplantation Assay, Control

Journal: Cell host & microbe

Article Title: Single Particle Imaging of Polarized Hepatoma Organoids upon Hepatitis C Virus Infection Reveals an Ordered and Sequential Entry Process

doi: 10.1016/j.chom.2018.02.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-SR-B1 , Novus Biologicals , Cat#NB400–101; RRID: AB_10107658.

Techniques: Control, Virus, Recombinant, Cell Recovery, Cell Viability Assay, Plasmid Preparation, shRNA, Software